6USM
Structure of nuclease domain of human parvovirus B19 non-structural protein 1 in complex with zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2013-09-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.690, 102.690, 134.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.000 - 3.370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MBP |
RMSD bond length | 0.010 |
RMSD bond angle | 1.212 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.955 | 48.000 |
High resolution limit [Å] | 3.370 | 3.370 |
Rmerge | 0.125 | |
Number of reflections | 11564 | 11564 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 96.8 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.2 M LiSo4 and sodium acetate tri-hydrate buffer of pH4.6 |