6USC

Structure of Human Intelectin-1 in complex with KO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 3
Unit cell lengths	118.145, 118.145, 118.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 1.590
R-factor	0.1594
Rwork	0.158
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wmq
Data reduction software	DIALS (1.9.2)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.240	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.140	0.893
Rmeas	0.142	0.938
Rpim	0.023	0.273
Number of reflections	73205	4888
<I/σ(I)>	16.9
Completeness [%]	99.2	91.2
Redundancy	35.8	10.3
CC(1/2)	0.999	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM bis-Tris, 25% PEG 3350