6URZ
Barrier-to-autointegration factor soaked in methanol: 1 of 14 in MSCS set
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.900, 41.900, 213.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.114 - 1.653 |
| R-factor | 0.1653 |
| Rwork | 0.162 |
| R-free | 0.20050 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.110 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.073 | 0.540 |
| Number of reflections | 23951 | 2309 |
| <I/σ(I)> | 13.71 | 2.47 |
| Completeness [%] | 99.9 | |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 80 mM Tris pH 8.5, 16% Ethanol, 10% PEG-1450 |
