6URA

Crystal structure of RUBISCO from Promineofilum breve

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	163.060, 162.740, 90.000
Unit cell angles	90.00, 109.57, 90.00

Refinement procedure

Resolution	77.206 - 2.170
R-factor	0.1901
Rwork	0.188
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ybv
RMSD bond length	0.012
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.206	2.240
High resolution limit [Å]	2.170	2.170
Number of reflections	114370	11307
<I/σ(I)>	5.6
Completeness [%]	98.0
Redundancy	3.8
CC(1/2)	0.989	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris pH 8.0, 30% Polyethylene glycol monomethyl ether 2000