6URA
Crystal structure of RUBISCO from Promineofilum breve
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.060, 162.740, 90.000 |
Unit cell angles | 90.00, 109.57, 90.00 |
Refinement procedure
Resolution | 77.206 - 2.170 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ybv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.018 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.206 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 114370 | 11307 |
<I/σ(I)> | 5.6 | |
Completeness [%] | 98.0 | |
Redundancy | 3.8 | |
CC(1/2) | 0.989 | 0.557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Tris pH 8.0, 30% Polyethylene glycol monomethyl ether 2000 |