6UR7

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-17
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97985
Spacegroup name	P 43 21 2
Unit cell lengths	78.824, 78.824, 210.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.730 - 2.709
R-factor	0.2214
Rwork	0.219
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	low resolution SAD model
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.760
High resolution limit [Å]	2.709	7.350	2.709
Rmerge	0.157	0.076	0.916
Rmeas	0.165	0.080	1.009
Rpim	0.050	0.025	0.411
Number of reflections	18880	1093	895
<I/σ(I)>	6.8
Completeness [%]	99.9	99.6	99.7
Redundancy	10.6	10.7	5.6
CC(1/2)		0.988	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Lithium sulfate, 0.1M Tris-Cl, 40% PEG400