6UR5
Resurfaced influenza hemagglutinin in complex with a broadly neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.270, 141.470, 166.870 |
Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
Resolution | 48.590 - 4.000 |
R-factor | 0.301 |
Rwork | 0.300 |
R-free | 0.32180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ha0 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.590 | 48.590 | 4.200 |
High resolution limit [Å] | 4.000 | 46.000 | 4.000 |
Rmerge | 0.290 | 0.041 | 0.734 |
Rmeas | 0.340 | 0.048 | 0.875 |
Total number of observations | 63077 | ||
Number of reflections | 17321 | 2 | 2162 |
<I/σ(I)> | 4.51 | 21.74 | 1.67 |
Completeness [%] | 97.5 | 14.3 | 89.6 |
Redundancy | 3.642 | 4 | 3.284 |
CC(1/2) | 0.950 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM sodium citrate (pH 4.5), 20% (wt/vol) PEG 4000 |