6UR2
DNA polymerase I Large Fragment from Bacillus stearothermophilus with DNA template and primer containing an N3'-> P5' linkage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2018-12-14 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.197, 93.679, 107.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.850 - 2.270 |
R-factor | 0.2313 |
Rwork | 0.229 |
R-free | 0.27670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l5u |
RMSD bond length | 0.008 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.270 | 2.280 |
Rmerge | 0.120 | 0.508 |
Rmeas | 0.129 | 0.543 |
Rpim | 0.046 | 0.192 |
Number of reflections | 39951 | 3928 |
<I/σ(I)> | 17.9 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8 | 8 |
CC(1/2) | 0.991 | 0.986 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10mM MgCl2, 0.8 M Succinic acid pH 7.0 |