6UQ3

RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97665
Spacegroup name	C 1 2 1
Unit cell lengths	169.189, 222.584, 194.612
Unit cell angles	90.00, 101.34, 90.00

Refinement procedure

Resolution	49.268 - 3.470
R-factor	0.235
Rwork	0.234
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6blo
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	3.530
High resolution limit [Å]	3.470	3.470
Rmerge	0.580	2.776
Rmeas	0.626	2.990
Rpim	0.235	1.105
Total number of observations	636868	32397
Number of reflections	91067	4503
<I/σ(I)>	4.9	0.9
Completeness [%]	100.0	100
Redundancy	7	7.2
CC(1/2)	0.950	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000