6UQ2

RNA polymerase II elongation complex with dG in state 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	C 1 2 1
Unit cell lengths	165.688, 223.317, 193.516
Unit cell angles	90.00, 99.37, 90.00

Refinement procedure

Resolution	49.754 - 3.200
R-factor	0.2305
Rwork	0.230
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6blo
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.754	3.140
High resolution limit [Å]	3.090	3.090
Rmerge	0.333	4.636
Rmeas	0.357	4.969
Rpim	0.128	1.784
Total number of observations		48106
Number of reflections	126625	6215
<I/σ(I)>	7.6	0.6
Completeness [%]	100.0	100
Redundancy	7.7	7.7
CC(1/2)	0.992	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000