6UPZ
RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.664, 222.901, 193.486 |
| Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
| Resolution | 49.039 - 3.100 |
| R-factor | 0.2249 |
| Rwork | 0.224 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blo |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 3.070 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.356 | 3.318 |
| Rmeas | 0.381 | 3.555 |
| Rpim | 0.137 | 1.272 |
| Total number of observations | 1050776 | 51823 |
| Number of reflections | 136357 | 6691 |
| <I/σ(I)> | 7.7 | 0.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.7 | 7.7 |
| CC(1/2) | 0.986 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
