6UPY

RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 2E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	168.536, 223.125, 193.062
Unit cell angles	90.00, 100.92, 90.00

Refinement procedure

Resolution	49.218 - 3.400
R-factor	0.2283
Rwork	0.227
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6blo
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	3.350
High resolution limit [Å]	3.290	3.290
Rmerge	0.450	4.436
Rmeas	0.482	4.750
Rpim	0.173	1.694
Total number of observations	816457	40606
Number of reflections	105549	5201
<I/σ(I)>	6	0.7
Completeness [%]	99.6	99.3
Redundancy	7.7	7.8
CC(1/2)	0.986	0.278

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000