6UPY
RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 2E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-09-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.536, 223.125, 193.062 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 49.218 - 3.400 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.28240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blo |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.220 | 3.350 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.450 | 4.436 |
| Rmeas | 0.482 | 4.750 |
| Rpim | 0.173 | 1.694 |
| Total number of observations | 816457 | 40606 |
| Number of reflections | 105549 | 5201 |
| <I/σ(I)> | 6 | 0.7 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 0.986 | 0.278 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
