6UP4

Crystal structure of the murine DHX36 helicase in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-04
Detector	RAYONIX MX300-HS
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	66.160, 115.930, 132.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.826 - 2.400
R-factor	0.1887
Rwork	0.186
R-free	0.23570
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3374)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.826	2.460
High resolution limit [Å]	2.400	2.400
Rmeas	0.120	1.520
Number of reflections	77042	2698
<I/σ(I)>	10.8	1.6
Completeness [%]	100.0
Redundancy	3.9
CC(1/2)	1.000	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	10% PEG 5000 MME, 0.1 M HEPES pH 7.0, 5% v/v Tacsimate