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6UP0

Structure of the Mango-III fluorescent aptamer bound to YO3-Biotin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2018-08-24
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)1.0
Spacegroup nameP 21 21 21
Unit cell lengths62.284, 66.921, 76.628
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.410 - 2.800
R-factor0.1777
Rwork0.174
R-free0.21460
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6e8s
RMSD bond length0.006
RMSD bond angle1.198
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.14_3211)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.4102.900
High resolution limit [Å]2.7202.800
Rmerge1.000
Number of reflections166041351
<I/σ(I)>27.6
Completeness [%]99.9
Redundancy6.3
CC(1/2)0.3400.340
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP729440 mM sodium cacodylate, 0.08 M sodium chloride, 0.012 M potassium chloride, 0.02 M magnesium chloride, 0.012 M spermine, 5.5% sucrose, 31% MPD

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