6UP0
Structure of the Mango-III fluorescent aptamer bound to YO3-Biotin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.284, 66.921, 76.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.410 - 2.800 |
R-factor | 0.1777 |
Rwork | 0.174 |
R-free | 0.21460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e8s |
RMSD bond length | 0.006 |
RMSD bond angle | 1.198 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.410 | 2.900 |
High resolution limit [Å] | 2.720 | 2.800 |
Rmerge | 1.000 | |
Number of reflections | 16604 | 1351 |
<I/σ(I)> | 27.6 | |
Completeness [%] | 99.9 | |
Redundancy | 6.3 | |
CC(1/2) | 0.340 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 40 mM sodium cacodylate, 0.08 M sodium chloride, 0.012 M potassium chloride, 0.02 M magnesium chloride, 0.012 M spermine, 5.5% sucrose, 31% MPD |