6UOU
MicroED structure of OsPYL/RCAR5 (24-29) at 9 e-/A^2
Experimental procedure
| Source type | ELECTRON MICROSCOPE |
| Source details | Technai F30 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-04-04 |
| Detector | TemCam-XF416 |
| Wavelength(s) | 0.0197 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 4.730, 11.360, 39.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 1.040 - 1.040 |
| R-factor | 0.2498 |
| Rwork | 0.249 |
| R-free | 0.24970 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 7.462 | 7.462 | 1.100 |
| High resolution limit [Å] | 1.000 | 8.000 | 1.000 |
| Rmerge | 0.253 | 0.691 | |
| Rmeas | 0.270 | 0.733 | |
| Total number of observations | 12269 | ||
| Number of reflections | 1339 | 312 | |
| <I/σ(I)> | 4.12 | 2.13 | |
| Completeness [%] | 97.4 | 98.1 | |
| Redundancy | 9.163 | 9.702 | |
| CC(1/2) | 0.970 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% ethanol |
