6UOU

MicroED structure of OsPYL/RCAR5 (24-29) at 9 e-/A^2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	Technai F30
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-04
Detector	TemCam-XF416
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.730, 11.360, 39.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	1.040 - 1.040
R-factor	0.2498
Rwork	0.249
R-free	0.24970
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.462	7.462	1.100
High resolution limit [Å]	1.000	8.000	1.000
Rmerge	0.253		0.691
Rmeas	0.270		0.733
Total number of observations	12269
Number of reflections	1339		312
<I/σ(I)>	4.12		2.13
Completeness [%]	97.4		98.1
Redundancy	9.163		9.702
CC(1/2)	0.970		0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol