6UOS

MicroED structure of OsPYL/RCAR5 (24-29) at 6 e-/A^2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	Technai F30
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-04
Detector	TemCam-XF416
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.720, 11.280, 39.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.418 - 0.900
R-factor	0.2284
Rwork	0.227
R-free	0.24360
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.418	7.418	1.000
High resolution limit [Å]	0.900	8.000	0.900
Rmerge	0.202		0.666
Rmeas	0.216		0.706
Total number of observations	16619
Number of reflections	1792		478
<I/σ(I)>	5.57		2.51
Completeness [%]	97.8		99.4
Redundancy	9.274		9.958
CC(1/2)	0.982		0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol