6UOR

MicroED structure of OsPYL/RCAR5 (24-29) at 3 e-/A^2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	Technai F30
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-04
Detector	TemCam-XF416
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.730, 11.320, 38.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.380 - 0.900
R-factor	0.2086
Rwork	0.206
R-free	0.23970
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.380	7.380	1.000
High resolution limit [Å]	0.900	8.000	0.900
Rmerge	0.186		0.405
Rmeas	0.198		0.430
Total number of observations	15449
Number of reflections	1776		468
<I/σ(I)>	6.76		4.01
Completeness [%]	97.7		99.4
Redundancy	8.699		9.288
CC(1/2)	0.987		0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol