6UOM
Y271G DNA polymerase beta ternary complex with templating adenine and incoming r8-oxo-GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-23 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.800, 80.400, 55.500 |
Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.050 |
R-factor | 0.1985 |
Rwork | 0.194 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.088 | 25.000 | 2.090 |
High resolution limit [Å] | 2.040 | 5.550 | 2.050 |
Rmerge | 0.079 | 0.037 | 0.650 |
Rmeas | 0.093 | 0.044 | 0.809 |
Rpim | 0.047 | 0.023 | 0.474 |
Number of reflections | 37845 | 1343 | 1236 |
<I/σ(I)> | 8 | ||
Completeness [%] | 99.1 | 98.7 | 95.8 |
Redundancy | 3.6 | 3.3 | 2.2 |
CC(1/2) | 0.993 | 0.468 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 50 mM imidazole, 350 mM sodium chloride, 19% PEG3350, pH 8.0 |