6UNT
Barrier-to-autointegration factor soaked in DMSO: 1 of 14 in MSCS set
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 277.15 |
Detector technology | CCD |
Collection date | 2011-08-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 41.770, 41.770, 213.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.994 - 1.750 |
R-factor | 0.1715 |
Rwork | 0.166 |
R-free | 0.22260 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.210 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.101 | 1.075 |
Rmeas | 0.105 | 1.116 |
Rpim | 0.029 | 0.297 |
Total number of observations | 276443 | |
Number of reflections | 20320 | 1075 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.6 | 13.9 |
CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 80 mM Tris pH 8.5, 16% Ethanol, 10% PEG-1450 |