6UN6
Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-NO2Y); ih circular permutant (50-51)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.947, 67.719, 58.363 |
Unit cell angles | 90.00, 102.28, 90.00 |
Refinement procedure
Resolution | 38.530 - 1.500 |
R-factor | 0.161 |
Rwork | 0.159 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ofk |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13RC2_2986) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.528 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.073 | 2.238 |
Number of reflections | 57467 | 4040 |
<I/σ(I)> | 16.7 | 1.11 |
Completeness [%] | 98.1 | 93 |
Redundancy | 13.26 | 12.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15 M ammonium acetate, 34% PEG 3350 |