6UN4
Crystal structure of rsEGFP2, Y67(3-ClY), Y107(3-ClY)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.119499 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.341, 61.848, 69.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.529 - 1.499 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.19750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pfr |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13rc2_2986) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.529 | 34.529 | 1.540 |
High resolution limit [Å] | 1.499 | 6.700 | 1.500 |
Rmerge | 0.053 | 0.017 | 1.874 |
Rmeas | 0.055 | 0.017 | 1.949 |
Number of reflections | 35265 | 460 | 2510 |
<I/σ(I)> | 24.27 | 106.23 | 1.32 |
Completeness [%] | 98.1 | 98.5 | 95.4 |
Redundancy | 13.247 | 12.348 | 13.119 |
CC(1/2) | 1.000 | 1.000 | 0.675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 80 mM HEPES, pH 8.1, 1.84 M ammonium sulfate |