6UMW

Crystal structure of hEphB1 bound with chlortetracycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	32.932, 91.090, 96.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.226 - 1.982
R-factor	0.1836
Rwork	0.181
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zfx
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.160	0.590
Number of reflections	21042	2074
<I/σ(I)>	17.9
Completeness [%]	99.8
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium malonate, pH 4.6, 14% PEG3350