6UMB
Crystal structure of TRIM7 B30.2 domain at 1.8 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.360, 67.440, 85.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.800 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.685 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.420 | 50.420 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.020 | 1.800 |
| Rmerge | 0.076 | 0.034 | 0.546 |
| Rmeas | 0.084 | 0.038 | 0.598 |
| Rpim | 0.035 | 0.015 | 0.240 |
| Total number of observations | 193627 | 1815 | 11498 |
| Number of reflections | 33860 | 328 | 1943 |
| <I/σ(I)> | 10.7 | 18.8 | 2.9 |
| Completeness [%] | 99.6 | 98.8 | 97.8 |
| Redundancy | 5.7 | 5.5 | 5.9 |
| CC(1/2) | 0.995 | 0.999 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | Sodium malonate pH 6.0 |
