6UMA
Crystal structure of the TRIM7 B30.2 domain at 1.6 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.940, 62.070, 128.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.690 - 1.600 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.780 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.690 | 20.690 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.620 | 1.600 |
| Rmerge | 0.042 | 0.027 | 0.337 |
| Rmeas | 0.046 | 0.030 | 0.371 |
| Rpim | 0.019 | 0.012 | 0.152 |
| Total number of observations | 237684 | 1861 | 10861 |
| Number of reflections | 44099 | 327 | 2055 |
| <I/σ(I)> | 11.3 | 25.3 | 1.7 |
| Completeness [%] | 97.6 | 93.3 | 90.5 |
| Redundancy | 5.4 | 5.7 | 5.3 |
| CC(1/2) | 0.999 | 0.999 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | Sodium malonate pH 6.0, glycerol |
