6ULI
Molecular basis for tumor infiltrating TCR recognition of hotspot KRAS-G12D mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.365, 76.266, 62.696 |
| Unit cell angles | 90.00, 118.22, 90.00 |
Refinement procedure
| Resolution | 38.130 - 1.880 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nt6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.133 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.095 | 0.630 |
| Number of reflections | 31888 | 1534 |
| <I/σ(I)> | 42.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris pH 6.5, 0.05M CaCl2 dihydrate, 30% PEG MME 500 |
