6UL3
Crystal structure of a lysozyme from Litopenaeus vannamei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-10-08 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.990, 59.321, 100.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.740 - 3.000 |
R-factor | 0.2 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lys |
RMSD bond length | 0.005 |
RMSD bond angle | 1.087 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17-3344-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.740 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 4921 | 4921 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 91.5 | |
Redundancy | 2.2 | |
CC(1/2) | 0.980 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.1M Tris pH 8.5, 25% (w/v) tert-Butanol |