6UKY
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.770, 110.770, 35.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.390 - 1.950 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | pdbid 6UKM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data scaling software | Aimless (0.5.28) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.390 | 1.956 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.126 | 1.348 |
| Rmeas | 0.132 | 1.090 |
| Rpim | 0.037 | 0.299 |
| Total number of observations | 31524 | |
| Number of reflections | 16545 | 2404 |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 12.7 | 13.3 |
| CC(1/2) | 0.996 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
