6UKW
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.440, 109.520, 59.320 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 25.390 - 1.970 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6UKM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data scaling software | Aimless (0.7.1) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.012 | 2.008 |
| High resolution limit [Å] | 1.970 | 1.974 |
| Rmerge | 0.120 | 0.834 |
| Rmeas | 0.143 | 0.841 |
| Rpim | 0.077 | 0.449 |
| Total number of observations | 13409 | |
| Number of reflections | 31056 | 4160 |
| <I/σ(I)> | 8.7 | 4.1 |
| Completeness [%] | 95.5 | 97.7 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.987 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
