6UKU
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.970, 73.180, 37.050 |
| Unit cell angles | 90.00, 102.32, 90.00 |
Refinement procedure
| Resolution | 18.890 - 1.680 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6UKM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data scaling software | Aimless (0.6.2) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.481 | 1.711 |
| High resolution limit [Å] | 1.680 | 1.682 |
| Rmerge | 0.026 | 0.489 |
| Rmeas | 0.031 | 0.478 |
| Rpim | 0.017 | 0.252 |
| Total number of observations | 12977 | |
| Number of reflections | 23503 | 3704 |
| <I/σ(I)> | 19.5 | 2.4 |
| Completeness [%] | 91.1 | 99.2 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.999 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
