6UKI
HhaI endonuclease in Complex with DNA in space group P212121 (pH 6.0)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.411, 94.998, 97.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.970 - 2.700 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uke |
RMSD bond length | 0.004 |
RMSD bond angle | 0.689 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.980 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.216 | 1.749 |
Rpim | 0.078 | 0.757 |
Number of reflections | 45835 | 4545 |
<I/σ(I)> | 11.2 | 2.3 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 8.1 | 5.2 |
CC(1/2) | 0.505 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 10% v/v 2-propanol, 0.1 M MES/sodium hydroxide, pH 6.0, 0.2 M calcium acetate |