6UKG
HhaI endonuclease in Complex With DNA in space group P21 (pH 4.2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.849, 37.545, 69.923 |
Unit cell angles | 90.00, 110.26, 90.00 |
Refinement procedure
Resolution | 31.520 - 1.160 |
R-factor | 0.1412 |
Rwork | 0.141 |
R-free | 0.15720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uke |
RMSD bond length | 0.007 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.520 | 1.210 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmerge | 0.093 | 0.354 |
Rpim | 0.039 | 0.217 |
Number of reflections | 192216 | 12298 |
<I/σ(I)> | 14.5 | 3.6 |
Completeness [%] | 88.9 | 66.5 |
Redundancy | 6.1 | 3.2 |
CC(1/2) | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 10% v/v 2-propanol, sodium phosphate / citric acid, pH 4.2, 0.2 M lithium sulfate |