6UKE
HhaI endonuclease in Complex with Iodine-Labelled DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-20 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.890, 37.193, 68.744 |
| Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
| Resolution | 28.650 - 1.620 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20310 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.957 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.650 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.091 | 0.767 |
| Rpim | 0.021 | 0.413 |
| Number of reflections | 69465 | 2133 |
| <I/σ(I)> | 21.4 | 2.2 |
| Completeness [%] | 97.8 | |
| Redundancy | 17.8 | |
| CC(1/2) | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | 35% v/v 2-propanol, 0.1 M sodium phosphate dibasic / citric acid, pH 4.2 |
