6UKC

Crystal structure of a lysozyme from Litopenaeus vannamei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-24
Detector	RIGAKU
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	82.368, 82.368, 38.665
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.692 - 2.250
Rwork	0.200
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lsy
RMSD bond length	0.008
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	2.320
High resolution limit [Å]	2.250	2.250
Number of reflections	6270	6275
<I/σ(I)>	8.2
Completeness [%]	86.2
Redundancy	2.8
CC(1/2)	0.996	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298.15	HEPES 0.1M, pH 7.5, 20 (w/v) PEG 10,000%