6UJX
HIV-1 wild-type reverse transcriptase-DNA complex with (-)-FTC-TP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97395 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 169.804, 167.685, 103.309 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.598 - 2.705 |
R-factor | 0.199062841517 |
Rwork | 0.197 |
R-free | 0.24381 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kk1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.110 | 0.735 |
Number of reflections | 39743 | 3373 |
<I/σ(I)> | 12.8 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% PEG 4000, MES pH 6.0, 10mM magnesium sulfate |