6UJF
Crystal structure of the Clostridial cellulose synthase subunit Z (CcsZ) from Clostridioides difficile
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.020, 46.530, 61.090 |
Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
Resolution | 31.692 - 2.000 |
R-factor | 0.2369 |
Rwork | 0.234 |
R-free | 0.28170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uje |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MrBUMP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.692 | 2.071 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.018 | 0.284 |
Rmeas | 0.026 | 0.401 |
Rpim | 0.018 | 0.284 |
Number of reflections | 25294 | 2157 |
<I/σ(I)> | 17.96 | 2.4 |
Completeness [%] | 99.5 | 99.58 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 100 mM MES, 200 mM calcium acetate, 20% (v/v) poly(ethylene glycol) 8000, 2.5 mM cellotriose |