6UIO
Crystal structure of mouse CRES (Cystatin-Related Epididymal Spermatogenic)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.048, 85.924, 111.677 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.530 - 1.830 |
R-factor | 0.2282 |
Rwork | 0.224 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6roa |
RMSD bond length | 0.016 |
RMSD bond angle | 1.918 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.530 | 1.893 |
High resolution limit [Å] | 1.827 | 1.827 |
Rmerge | 0.082 | 0.842 |
Rmeas | 0.096 | 0.971 |
Rpim | 0.048 | 0.473 |
Number of reflections | 39480 | 3967 |
<I/σ(I)> | 17.15 | 2.22 |
Completeness [%] | 88.8 | |
Redundancy | 4.1 | |
CC(1/2) | 0.996 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 292 | 2.1M (NH4)2SO4, 0.2M NaI |