6UIG

Crystal structure of human monoclonal antibody H7.200 in complex with H7N9 hemagglutinin HA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 43 2 2
Unit cell lengths	156.493, 156.493, 229.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.821 - 3.200
R-factor	0.2085
Rwork	0.207
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n5j
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.821	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.097	0.607
Number of reflections	47601	6843
<I/σ(I)>	11.3
Completeness [%]	99.9
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M sodium citrate pH 6.0, 10% isopropanol, 22% PEG 400