6UID
Structure of the cytoplasmic domain of the T3SS sorting platform protein PscD from P. aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 48.086, 48.086, 235.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.999 - 1.450 |
R-factor | 0.1699 |
Rwork | 0.169 |
R-free | 0.19830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a0e |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | MoRDa |
Refinement software | PHENIX (1.14_3237) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.100 | 47.100 | 1.470 |
High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
Rmerge | 0.069 | 0.038 | 0.912 |
Total number of observations | 415491 | 3177 | 7489 |
Number of reflections | 29928 | 272 | 1343 |
<I/σ(I)> | 18.9 | 44.4 | 1.7 |
Completeness [%] | 99.6 | 99.9 | 95.4 |
Redundancy | 13.9 | 11.7 | 5.6 |
CC(1/2) | 0.999 | 1.000 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 291 | 1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic |