6UHZ

WDR5 in complex with Myc site fragment inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-14
Detector	MAR CCD 165 mm
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	134.374, 46.903, 112.462
Unit cell angles	90.00, 117.61, 90.00

Refinement procedure

Resolution	37.276 - 1.258
R-factor	0.1535
Rwork	0.152
R-free	0.17580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.854
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.280
High resolution limit [Å]	1.258	3.420	1.260
Rmerge	0.052	0.033	0.244
Rmeas	0.060	0.038	0.280
Rpim	0.029	0.019	0.136
Number of reflections	163606	8006	7910
<I/σ(I)>	12
Completeness [%]	97.4	92.2	94.4
Redundancy	4	3.8	3.9
CC(1/2)		0.998	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-Tris pH 6.0, 0.2 M ammonium acetate, 28% PEG3350