6UHZ
WDR5 in complex with Myc site fragment inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.374, 46.903, 112.462 |
Unit cell angles | 90.00, 117.61, 90.00 |
Refinement procedure
Resolution | 37.276 - 1.258 |
R-factor | 0.1535 |
Rwork | 0.152 |
R-free | 0.17580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.854 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.280 |
High resolution limit [Å] | 1.258 | 3.420 | 1.260 |
Rmerge | 0.052 | 0.033 | 0.244 |
Rmeas | 0.060 | 0.038 | 0.280 |
Rpim | 0.029 | 0.019 | 0.136 |
Number of reflections | 163606 | 8006 | 7910 |
<I/σ(I)> | 12 | ||
Completeness [%] | 97.4 | 92.2 | 94.4 |
Redundancy | 4 | 3.8 | 3.9 |
CC(1/2) | 0.998 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Bis-Tris pH 6.0, 0.2 M ammonium acetate, 28% PEG3350 |