6UHO

Crystal Structure of C148 mGFP-cDNA-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-22
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	64.710, 52.220, 86.440
Unit cell angles	90.00, 94.23, 90.00

Refinement procedure

Resolution	64.530 - 1.950
R-factor	0.2155
Rwork	0.214
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
RMSD bond length	0.010
RMSD bond angle	1.783
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.204	53.592	2.060
High resolution limit [Å]	1.950	6.170	1.950
Rmerge		0.056	0.406
Rmeas	0.107	0.065	0.464
Rpim	0.051	0.032	0.222
Total number of observations	179992
Number of reflections	41971	1414	6133
<I/σ(I)>	9.4	15.7	3.3
Completeness [%]	99.2	99.8	100
Redundancy	4.3	4.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1 microliter C148 mGFP-cDNA-2 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.15 M potassium bromide, 30% (w/v) PEG MME 2000) in a sitting drop with a 70 microliter reservoir (0.15 M potassium bromide, 30% (w/v) PEG MME 2000)