6UHN

Crystal Structure of C148 mGFP-cDNA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-30
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	64.920, 52.180, 86.469
Unit cell angles	90.00, 94.24, 90.00

Refinement procedure

Resolution	53.720 - 1.920
R-factor	0.2179
Rwork	0.216
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
RMSD bond length	0.011
RMSD bond angle	1.832
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.233	64.742	2.020
High resolution limit [Å]	1.920	6.070	1.920
Rmerge		0.073	0.442
Rmeas	0.129	0.085	0.507
Rpim	0.063	0.042	0.244
Total number of observations	192150
Number of reflections	44394	1486	6441
<I/σ(I)>	7.8	12.6	3.1
Completeness [%]	100.0	99.7	99.9
Redundancy	4.3	4.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	1 microliter C148 mGFP-cDNA-1 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.2 M lithium sulfate, 0.1 M Bis-Tris Buffer pH 5.5, 25% (w/v) PEG 3350) in a sitting drop with a 70 microliter reservoir 0.2 M lithium sulfate, 0.1 M Bis-Tris Buffer pH 5.5, 25% (w/v) PEG 3350)