6UHH
Crystal Structure of Human RYR Receptor 3 ( 848-1055) in Complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-11 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97927 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.043, 61.855, 279.128 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.521 - 3.138 |
R-factor | 0.2372 |
Rwork | 0.233 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uhb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.838 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.11.1-2575_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.190 |
High resolution limit [Å] | 3.138 | 8.510 | 3.140 |
Rmerge | 0.098 | 0.086 | 0.547 |
Rmeas | 0.118 | 0.106 | 0.660 |
Rpim | 0.065 | 0.061 | 0.363 |
Number of reflections | 18902 | 965 | 919 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 96.0 | 86.1 | 97.7 |
Redundancy | 3 | 2.7 | 3.1 |
CC(1/2) | 0.940 | 0.593 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.1 M Tris:HCl pH 8.5, 20% (w/v) PEG 1000, protein in co-crystallization with 10mM ATP |