6UHE
Closed-form Crystal Structure of Human RYR Receptor 3 ( 848-1055)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97927 |
Spacegroup name | P 65 |
Unit cell lengths | 64.673, 64.673, 469.779 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.340 - 2.892 |
R-factor | 0.2561 |
Rwork | 0.254 |
R-free | 0.30160 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.938 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.11.1-2575_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.890 | 8.000 | 2.950 |
Rmerge | 0.069 | 0.045 | 0.694 |
Rmeas | 0.079 | 0.052 | 0.800 |
Rpim | 0.038 | 0.025 | 0.391 |
Number of reflections | 24385 | 1138 | 1111 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 98.2 | 93.7 | 99.2 |
Redundancy | 4.1 | 4 | 3.8 |
CC(1/2) | 0.986 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 297 | 0.1 M Tris:HCl pH 8.5, 1.2 M Sodium Citrate Tribasic |