6UGI

Crystal structure of a fragment of E. coli tRNA(Asp) consisting of its acceptor stem/T stem-loop. Long unit cell.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12720
Spacegroup name	H 3 2
Unit cell lengths	43.519, 43.519, 281.795
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.380 - 1.750
R-factor	0.232
Rwork	0.231
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ugj
RMSD bond length	0.010
RMSD bond angle	1.913
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.380	1.872
High resolution limit [Å]	1.750	1.754
Rmerge	0.118	1.257
Rmeas	0.126	1.354
Rpim	0.045	0.494
Number of reflections	9226	3401
<I/σ(I)>	8.2
Completeness [%]	89.0	34.9
Redundancy	8.1	7.4
CC(1/2)	0.997	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	2 M ammonium sulfate