6UGG

Structure of unmodified E. coli tRNA(Asp)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0782
Spacegroup name	P 31 2 1
Unit cell lengths	64.677, 64.677, 195.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.620 - 1.950
R-factor	0.2164
Rwork	0.215
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ehz
RMSD bond length	0.003
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.620	2.056
High resolution limit [Å]	1.950	1.950
Rmerge	0.155	2.683
Rmeas	0.158	2.724
Rpim	0.029	0.468
Number of reflections	35638	55102
<I/σ(I)>	15.8	1.8
Completeness [%]	94.0	50.8
Redundancy	30	33.7
CC(1/2)	0.992	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	2 uL 20 mg/ml E. coli tRNA(Asp) + 2 uL equilibration solution (50 mM sodium cacodylate, pH 7.5, 30% MPD, 10 mM spermine tetrahydrochloride, 300 mM sodium chloride, 300 mM potassium chloride) + 0.5 uL 0.25% w/v p-coumaric acid, 0.25% w/v phenylurea, 0.25% w/v poly(3-hydroxybutyric acid), 0.25% w/v sulfaguanidine, 0.02 M sodium HEPES, pH 6.8