6UG5

Closed Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	P 1 21 1
Unit cell lengths	74.012, 87.421, 74.041
Unit cell angles	90.00, 110.56, 90.00

Refinement procedure

Resolution	36.974 - 2.357
R-factor	0.1765
Rwork	0.174
R-free	0.22410
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.116
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.400
High resolution limit [Å]	2.357	6.400	2.360
Rmerge	0.085	0.038	0.510
Rmeas	0.095	0.042	0.593
Rpim	0.042	0.018	0.296
Number of reflections	36401	1890	1711
<I/σ(I)>	11.2
Completeness [%]	99.4	99.8	93.3
Redundancy	5	5	3.7
CC(1/2)		0.996	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	1.5M sodium citrate