6UG4

Open Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	P 41 21 2
Unit cell lengths	84.201, 84.201, 229.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.101 - 2.295
R-factor	0.1992
Rwork	0.197
R-free	0.23540
Structure solution method	SAD
RMSD bond length	0.050
RMSD bond angle	1.414
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.111	0.081	0.725
Total number of observations	321203
Number of reflections	41451	2177	2074
<I/σ(I)>	9.8
Completeness [%]	96.2	90.3	98.1
Redundancy	7.7	8.9	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	0.1 M Bis-Tris Propane:NaOH pH 7.0, 2.5 M Ammonium Sulfate