6UFW
Crystal structure of the CBM3 from Bacillus subtilis at 1.28 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.092, 55.176, 89.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.727 - 1.280 |
| R-factor | 0.1478 |
| Rwork | 0.146 |
| R-free | 0.17310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.727 | 1.325 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 31335 | 2914 |
| <I/σ(I)> | 28.27 | |
| Completeness [%] | 95.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20 % (w/v) PEG3350, 0.20 M potassium thiocynate |
