6UFM
Cocrystal Structure of the Nocardia farcinica ileS T-box riboswitch in complex with its cognate tRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 A |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 42.230, 93.980, 179.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.990 - 2.820 |
R-factor | 0.195 |
Rwork | 0.191 |
R-free | 0.23270 |
Structure solution method | SAD |
Starting model (for MR) | tRNA-Gly |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.990 | 2.920 |
High resolution limit [Å] | 2.820 | 2.820 |
Rmerge | 0.080 | 1.960 |
Number of reflections | 17899 | 1726 |
<I/σ(I)> | 15.2 | 1.2 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 13 | 10.3 |
CC(1/2) | 0.994 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 20-25 % PEG 3350, 0.1 M Bis-Tris, pH 6.5, 0.2 M Li2SO4, 10 mM MgCl2, 1 mM Spermine |