6UFK

Pistol ribozyme product crystal soaked in Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.89170
Spacegroup name	P 42 21 2
Unit cell lengths	90.346, 90.346, 121.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.929 - 3.200
R-factor	0.2156
Rwork	0.211
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.400	3.150
High resolution limit [Å]	2.970	2.970
Rpim	0.052	33.100
Number of reflections	9562	9982
<I/σ(I)>	11.3
Completeness [%]	99.2
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	grown in 0.05 M HEPES sodium, pH 7.5, 0.1 M magnesium chloride, 25-30% MPD, soaked in 0.05 M HEPES sodium, pH 7.5, 0.1 M manganese(II) chloride, 35% MPD