6UFJ

Pistol ribozyme product crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 42 21 2
Unit cell lengths	90.734, 90.734, 121.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.493 - 2.645
R-factor	0.195
Rwork	0.190
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.645	2.650
Rpim	0.015	0.280
Number of reflections	15346	723
<I/σ(I)>	43.1
Completeness [%]	99.8
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.05 M HEPES sodium, pH 7.5, 0.1 M magnesium chloride, 25-30% MPD